Carbohydrate assimilation profiles of Brazilian Candida dubliniensis isolates based on ID 32C system.
نویسندگان
چکیده
The purpose of the present study was to evaluate the identification of 19 Brazilian C. dubliniensis based on the biochemical profile exhibited when tested by the commercial identification kit ID 32C (bioMerieux). Thirteen of the isolates were rigorously identified as C. dubliniensis and the remaining isolates (six) were considered as having a doubtful profile but the software also suggested that there was 83.6% of chances for them to be C. dubliniensis. As well as pointed by the literature the identification obtained by phenotypic tests should be considered presumptive for C. dubliniensis due to variability of this new species.
منابع مشابه
Molecular Identification and In-Vitro Susceptibility of Candida albicans and C. dubliniensis Isolated from Immunocompromised Patients
Background: Yeasts are increasingly implicated in serious systemic infections. The aim of this study was to identify Candida albicans and C. dubliniensis from isolates of immunocompromised patients and evaluate the in vitro antifungal activities of them against antifungal agents. Methods: One hundred and seventy eight C. albicans were isolated by routine methods from 403 immunocompromised patie...
متن کاملDistinctive carbohydrate assimilation profiles used to identify the first clinical isolates of Candida dubliniensis recovered in the United States.
The conventional identification (assimilation profiles in the API 20C [bioMérieux Vitek, Inc., Hazelwood, Mo.] identification system, colony color on CHROMagar Candida [CHROMagar, Paris, France], and morphology on cornmealTween 80 agar) of 32 atypical isolates of Candida albicans recovered during a study of oropharyngeal candidiasis in human immunodeficiency virus-infected individuals could not...
متن کاملRapid differentiation of Candida dubliniensis from Candida albicans by early D-xylose assimilation.
OBJECTIVE To determine if D-xylose (XYL) and/or α-methyl-D-glucoside (MDG) assimilation can be used reliably as a rapid test to differentiate Candida dubliniensis from Candida albicans at an earlier time point such as 2 h after inoculation. MATERIALS AND METHODS Thirty isolates of C. albicans and C. dubliniensis recovered from anatomical sites and clinical specimens were used. Isolates were i...
متن کاملComparison of use of phenotypic and genotypic characteristics for identification of species of the anamorph genus Candida and related teleomorph yeast species.
A total of 49 type and neotype isolates and 32 clinical isolates of the anamorph genus Candida and related teleomorph genera were obtained from different culture collections and clinical laboratories. Isolates were subjected to two phenotypic methods of identification, Vitek yeast biochemical card (YBC) and API ID 32C, both based on carbohydrate assimilation, and one genotypic method, PCR finge...
متن کاملPerformance comparison of phenotypic and molecular methods for detection and differentiation of Candida albicans and Candida dubliniensis
BACKGROUND Candida albicans is the most pathogenic Candida species but shares many phenotypic features with Candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. Candidemia cases due to C. dubliniensis are increasingly being reported in recent years. Accurate identification is warranted since mortality rates are highest for C. albicans infections, howe...
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ورودعنوان ژورنال:
- Revista do Instituto de Medicina Tropical de Sao Paulo
دوره 47 2 شماره
صفحات -
تاریخ انتشار 2005